Medical Research Core Facilities & Platforms

The Medical Research Core Facility and Platforms serve the MNG-HA scientific community’s needs for reagents, assays, samples and data analysis for the conduct of research projects.

The Medical Research Core Facility and Platforms is comprised of the following units: Cell & Tissue Culture, Biochemical & Molecular Biology, Drug Discovery, Immunology, Light & Electron Microscopy and Mass Spectrometry and Medicinal Chemistry. Each unit has specific purpose and contribution to support the researchers.

To provide researchers with:

• Customized Research Reagents: Cell lines, Proteins etc.
• Services in Cell Culture, Proteomics & Metabolomics, Cell Imaging, Microscopy, Drug Screening, Medicinal Chemistry, Analytical Chemistry, Protein Expression etc.
• Laboratory space fully equipped with the latest research platforms
• Assist researchers in the conduct of their biomedical research in a safe environment.

• Drug Discovery
• Cancer Research
• Rare Diseases Research
• Cardiovascular Research

• Cell Culture Facility
  • Primary and Cell lines Culture
  • Stem Cell Differentiations
  • Stable Cell line generation
  • Transfection
  • PBMC Isolation

• Biochemistry, Microbiology and Molecular Biology Lab
  • Plasmid/DNA/RNA/Protein Extraction and their bioanalysis (western blot, gel electrophoresis)
  • PCR, PC-RFLP, qPCR
  • Recombinant protein Expression & Purification

• Lentivirus and Baculovirus Lab
  • Lentivirus for shRNA expression for gene knock-down
  • Lentivirus for gene overexpression
  • Lentivirus for cell stable-labeling

• Bio-Imaging Suite
  • Life Technologies EVOS FL system equipped with life cell imaging capability
  • Molecular Devices Image Express High Content Imaging System
  • Imaging using Image Express (Molecular Devices)
  • Jeol JEM-1400 Transmission Electron Microscope (TEM)
  • FEI Nova SEM Scanning Electron Microscope (SEM)
  • Zeiss LSM 780 Laser Scanning Confocal Microscope (cLSM) with life cell imaging capability
  • Nikon Total Internal Reflection Fluorescence Microscope system (TIRF)
  • Zeiss Laser Capture Microdissection (LCM) system equipped with PALM Micro Tweezers and life cell imaging capability

• Immunology and Flow-Cytometry Platform
  • Fluorescence-activated cell analysis and sorting
  • BD FACS Canto II: Quantitative and qualitative analysis and characterization of cells, molecules and particles
  • MoFlo XDP Cell Sorter: Cell Analysis and Isolation from human or animal model.
  • Bio-Plex® 3D Suspension Array System: High-throughput testing for nucleic acid and protein applications
  • Cell Culture: Services for culture of different cell lines available in Core Lab department

• Mass Spectrometry
  • Discovery and Targeted Proteomics
  • Discovery and Targeted Metabolomics
  • Molecular weight determination
  • Analysis of compounds for structural elucidation
  • Impurity Analysis

• Drug Discovery, Medicinal & Analytical Chemistry
  • Design and Synthesis of Biologically Active Compounds
  • Optimization of Drug Candidates and SAR Development
  • Purification and Identification of Small Molecule
  • Antibody Drug Conjugates Preparation
  • PET Tracer Design
  • Consultancy on API Synthesis
  • Assay Development
  • Lentivirus based shRNA
  • Plate readers: Fluorescence, FRET, Luminescence etc.
  • Disease specific assay: Reporters, Colony Formation, Primary Cell Culture, Stable Cell line generations, etc.

• Antibody Generation – Coming Soon